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Item 1 of 4
Item 1 of 4
Mouse Dyrk2 Antibody (C-term)
Catalog Number: orb1936159
| Catalog Number | orb1936159 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34467 |
| Tested applications | IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:1000, WB: 1:8000, IHC-P: 1:100 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 66556 Da |
| Target | This Mouse Dyrk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-549 amino acids from the C-terminal region of mouse Dyrk2. |
| UniProt ID | Q5U4C9 |
| NCBI | NP_001014412.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Dual specificity tyrosine-phosphorylation-regulate Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Mouse Dyrk2 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the Dyrk2 antibody detected the Dyrk2 protein (arrow).
Staining Dyrk2 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes: Anti-Dyrk2 Antibody (C-term) at 1:2000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Ba/F3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Mouse Dyrk2 Antibody (C-term) at 1: 8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: SK-OV-3 whole cell lysate. Lane 4: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
