Mouse Dyrk2 Antibody (C-term)

SKU: orb1936159

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:100
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD56689
• Immunogen: This Mouse Dyrk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-549 amino acids from the C-terminal region of mouse Dyrk2. Antigen Region: 521-549 aa.
• Target: Dyrk2 {ECO:0000312|MGI:MGI:1330301}
• Molecular Weight: 66556 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dual specificity tyrosine-phosphorylation-regulated kinase 2, Dyrk2 {ECO:0000312|MGI:MGI:1330301}

Mouse Dyrk2 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the Dyrk2 antibody detected the Dyrk2 protein (arrow).

Staining Dyrk2 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-Dyrk2 Antibody (C-term) at 1:2000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Ba/F3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mouse Dyrk2 Antibody (C-term) at 1: 8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: SK-OV-3 whole cell lysate. Lane 4: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

Dyrk2 {ECO:0000312|MGI:MGI:1330301}

UniProt

Q5U4C9

RefSeq (Protein):NP_001014412.1