Mouse Ctr9 Antibody (C-term)

• Catalog Number: orb1788078
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Mouse Ctr9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1022-1056 amino acids from the C-terminal region of Mouse Ctr9.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: 1022-1056 aa
• UniProt ID: Q62018
• MW: 133408 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RNA polymerase-associated protein CTR9 homolog, SH
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded M. colon section using Mouse Ctr9 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. testis section using Mouse Ctr9 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of A431 cells stained with Mouse Ctr9 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-Ctr9 Antibody (C-term) at 1:1000 dilution. Lane 1: HT-29 whole cell lysates. Lane 2: Jurkat whole cell lysates. Lane 3: mouse cerebellum lysates. Lane 4: mouse testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 133 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.