Mouse Cldn6 Antibody (C-term)

SKU: orb1926621

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Microbiology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:1000, FC - 1:25
• Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB51201
• Immunogen: This Mouse Cldn6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 186-229 amino acids from the C-terminal region of mouse Cldn6. Antigen Region: 186-229 aa.
• Target: Cldn6
• Molecular Weight: 23388 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Claudin-6, Skullin, Cldn6

Western blot analysis of lysate from mouse kidney tissue lysate, using Cldn6 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Overlay histogram showing colon26 cells stained (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

Cldn6

UniProt

Q9Z262