Mouse Skeletal Troponin I Monoclonal Antibody

SKU: orb2281299

Description

This Mouse Skeletal Troponin I Monoclonal Antibody is specifically designed for the detection of skeletal Troponin I. It is validated for use in Western Blot (WB) and Immunohistochemistry (IHC) on FFPE tissues. The antibody reacts with both human and mouse samples, specifically targeting the fast-twitch skeletal muscle isoform. Supplied as a liquid in PBS with 0.09% sodium azide at a concentration of 1000 µg/ml. For research use only.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB : 1:1,000； IHC : 1:100 - 1:500. ( Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. )
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: FI-23
• Immunogen: Human Skeletal Troponin I
• Target: Skeletal Troponin I
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMCD2B;DA2B;FSSV;fsTnI;troponin I fast twitch 2;troponin I type 2 (skeletal, fast);troponin I, fast skeletal muscle;troponin I, fast-twitch isoform;troponin I, fast-twitch skeletal muscle isoform;troponin I, skeletal, fast

Detection of human Skeletal Troponin I by western blot. Samples: Whole cell lysate (50 µg) from human skeletal muscle tissue lysate and HEK293T cell lysate. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds

Detection of human Skeletal Troponin I by immunohistochemistry. Sample: FFPE section of human skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody. Secondary: HRP-conjugated goat anti-mouse IgG

Detection of mouse Skeletal Troponin I by immunohistochemistry. Sample: FFPE section of mouse skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody. Secondary: HRP-conjugated goat anti-mouse IgG

Quick Database Links

Gene Symbol

Skeletal Troponin I

UniProt

P48788

RefSeq (Protein):NP_003273.1