Mouse p53 Monoclonal Antibody

SKU: orb1520134

Description

p53 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution Range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:50 - 1:200. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: DO-1
• Immunogen: Recombinant full length protein corresponding to Human p53 (N terminal). Epitope is within aa 20-25
• Target: p53
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

antigen NY-CO-13; BCC7; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; Tumor suppressor p53; tumor supressor p53

Detection of human p53 in FFPE colon carcinoma by immunohistochemsitry. Antibody: Mouse monoclonal anti-p53 [DO-1] (orb1520134). Secondary:HRP-conjugated goat anti-mouse IgG.Substrate:DAB.

Quick Database Links

Gene Symbol

p53

UniProt

P04637

RefSeq (Protein):NP_000537.3