Mouse anti Human IgG4 (pFc subclass specific)

• Catalog Number: orb22277
• Category: Antibodies
• Description: Monoclonal mouse antibody to human IgG4, subclass specific. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NI 315 (HP 6206)
• Tested applications: ELISA, ICC, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Secondary Antibody
• Form/Appearance: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 2.0 mg/ml. No foreign proteins added.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
• Source: Highly purified monoclonal IgG4 isolated from human serum
• RRID: AB_10754013
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 2.0 mg/ml. No foreign proteins added.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.