Mouse anti Human CD54, conjugated with Biotin

• Catalog Number: orb1570846
• Category: Antibodies
• Description: Mouse anti Human CD54, conjugated with Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: D3.6
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: CD54=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with the major histocompatibility complex (MHC) class II-negative human b-lymphoblastoid cell line 6.16c13
• Concentration: Titered for flow cytometry
• Form/Appearance: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Conjugation: Biotin
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
• UniProt ID: P05362
• Storage: Product should be stored at 4-8°C. DO NOT FREEZE
• Buffer/Preservatives: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Alternative names: CD54Biotin
• Note: For research use only
• Application notes: PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20º C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Expiration Date: 12 months from date of receipt.