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Catalog Number | orb21181 |
---|---|
Category | Antibodies |
Description | Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human Ig kappa chain, free and bound. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining and ELISA and immunoblotting using an indirect technique with monoclonal anti TRITC labelled with peroxidase. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NI 285, NI 250 |
Isotype | IgG1 |
Antibody Type | Secondary Antibody |
Form/Appearance | Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Conjugation | TRITC |
Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information. |
Source | Highly purified Bence Jones kappa proteins isolated from pooled human urine. |
RRID | AB_10752293 |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day. |
Buffer/Preservatives | Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |