Mouse Chicken IgY Light Chain Monoclonal Antibody

SKU: orb2281290

Description

Mouse Chicken IgY Light Chain Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : WB , ApplicationDilutionRange : 1:1,000
• Reactivity: Gallus
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 1Y-263
• Immunogen: Chicken IgY Light Chain
• Target: Chicken IgY Light Chain
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection of human NF-H using mouse anti-chicken IgY secondary antibody by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, OVCAR-8, HEK293T, SK-N-BE(2), and HeLa cells prepared using NETN lysis buffer. Primary: Chicken anti-NF-H antibody. Secondary: mouse anti-Chicken IgY Light Chain monoclonal antibody used at 1:1000. Tertiary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 1 second

Detection of human NF-H using mouse anti-chicken IgY secondary antibody by immunohistochemistry. Sample: FFPE section of human cerebellum. Primary: Chicken anti-NF-H antibody. Secondary: Mouse anti-Chicken IgY Light Chain monoclonal antibody

Detection of human NF-H using mouse anti-chicken IgY secondary antibody by immunocytochemistry. Sample: FFPE section of SK-N-BE(2) cells. Primary: Chicken anti-NF-H antibody. Secondary: Mouse anti-Chicken IgY Light Chain monoclonal antibody

Quick Database Links

Gene Symbol

Chicken IgY Light Chain

RefSeq:BDI00300.1