Mouse anti-Chicken IgY Light Chain Monoclonal Antibody

• Catalog Number: orb2281290
• Category: Antibodies
• Description: Mouse anti-Chicken IgY Light Chain Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1Y-263
• Tested applications: ICC, IHC, WB
• Reactivity: Gallus
• Immunogen: Chicken IgY Light Chain
• Antibody Type: Secondary Antibody
• Concentration: 500 µg/ml
• Dilution range: WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Conjugation: Unconjugated
• Target: Chicken IgY Light Chain
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) with 0.1% rAlbumin and 0.09% Sodium Azide
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human NF-H using mouse anti-chicken IgY secondary antibody by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, OVCAR-8, HEK293T, SK-N-BE(2), and HeLa cells prepared using NETN lysis buffer. Primary: Chicken anti-NF-H antibody. Secondary: mouse anti-Chicken IgY Light Chain monoclonal antibody used at 1:1000. Tertiary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 1 second

Detection of human NF-H using mouse anti-chicken IgY secondary antibody by immunohistochemistry. Sample: FFPE section of human cerebellum. Primary: Chicken anti-NF-H antibody. Secondary: Mouse anti-Chicken IgY Light Chain monoclonal antibody

Detection of human NF-H using mouse anti-chicken IgY secondary antibody by immunocytochemistry. Sample: FFPE section of SK-N-BE(2) cells. Primary: Chicken anti-NF-H antibody. Secondary: Mouse anti-Chicken IgY Light Chain monoclonal antibody