Mouse CD8 alpha Monoclonal Antibody

SKU: orb1520119

Description

CD8 alpha Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC
• Dilution Range: IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: C8/144B
• Immunogen: A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule
• Target: CD8 alpha
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 250 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD antigen CD8a; CD8; CD8 antigen, alpha polypeptide (p32); Leu2; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

Detection of human CD8a by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-CD8a monoclonal antibody [C8/144B]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human CD8 alpha (left) in human whole blood by flow cytometry. Antibody: Mouse anti-CD8 alpha monoclonal antibody [C8/144B] (orb1520119) or isotype control (right). Secondary: DyLight® 488-conjugated goat anti-mouse IgG. Analysis performed on lymphocyte gated population.

Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD8 alpha antibody [C8/144B] (orb1520119) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG.

Quick Database Links

Gene Symbol

CD8 alpha

UniProt

P01732

RefSeq (Protein):NP_741969.1