CD45RO Mouse Monoclonal Antibody

SKU: orb1520122

Description

CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 5 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:250
• Reactivity: Human
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: UCHL-1
• Immunogen: Cultured T-cells from an IL-2-dependent T-cell line
• Target: CD45RO
• Purity: The antibody was purified from cell culture supernatant
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B220; CD antigen CD45; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

Quick Database Links

Gene Symbol

CD45RO

UniProt

P08575

RefSeq (Protein):NP_563578.2