Mouse anti-CD45RO Monoclonal Antibody

• Catalog Number: orb1520122
• Category: Antibodies
• Description: CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UCHL-1
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Cultured T-cells from an IL-2-dependent T-cell line
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 5 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:250
• Form/Appearance: Whole IgG
• Purity: The antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: CD45RO
• UniProt ID: P08575
• NCBI: NP_002829.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: L-CA; protein tyrosine phosphatase, receptor type,
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human CD45RO by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat cells prepared using NETN lysis buffer. Antibody: mouse anti-CD45RO antibody used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes

Detection of human CD45RO by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD45RO antibody used at a dilution of 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: DAB. Counterstain: Hematoxylin (blue)

Detection of human CD45RO (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Mouse anti-CD45RO monoclonal antibody used at 1:250. Substrate: Opal™. Counterstain: DAPI (blue)

Detection of human CD45RO (shaded) in Jurkat cells by flow cytometry. Antibody: Mouse anti-CD45RO recombinant monoclonal or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG