Mouse anti-CD20 Monoclonal Antibody

• Catalog Number: orb1520131
• Category: Antibodies
• Description: CD20 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: L26
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Immunogen: Human tonsil B cells
• Antibody Type: Primary Antibody
• Concentration: 300 µg/ml
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD20
• UniProt ID: P11836
• NCBI: NP_690605.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Membrane-spanning 4-domains subfamily A member 1;
• Background: CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human CD20 by western blot. Samples: Whole cell lysate (25 µg) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 antibody used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue)

Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG

Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody used at 1:250. Secondary: DyLight® 594-conjugated goat anti-mouse IgG

Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 antibody used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue)