Mouse CD20 Monoclonal Antibody

SKU: orb1520131

Description

CD20 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: FC: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells. Immunohistochemistry (IHC): 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF): 1:250. WB: 1:1000
• Reactivity: Human
• Application Notes:
  All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: L26
• Immunogen: Human tonsil B cells
• Target: CD20
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 300 µg/ml
• Disclaimer: For research use only

Alternative Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD antigen CD20; CD20; CD20 antigen; CD20 receptor; CVID5; LEU-16; leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A2; S7

Detection of human CD20 by western blot. Samples: Whole cell lysate (25 µg) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 antibody used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue)

Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-mouse IgG

Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody used at 1:250. Secondary: DyLight® 594-conjugated goat anti-mouse IgG

Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 antibody used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue)

Quick Database Links

Gene Symbol

CD20

UniProt

P11836

RefSeq (Protein):NP_068769.2