Anti-Hu CD20 Purified

• Catalog Number: orb44418
• Category: Antibodies
• Description: Mouse Monoclonal to CD20.
• Clonality: Monoclonal
• Clone Number: MEM-97
• Tested applications: FC, IP
• Reactivity: Bovine, Human
• Isotype: Mouse IgG1
• Immunogen: Raji human Burkitt's lymphoma cell line
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD20
• Entrez: 931
• UniProt ID: P11836
• RRID: AB_10989823
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD20 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 10 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.

Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control (PPV-06; cat. no. orb43531) Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97).

Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD20 (MEM-97) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD20 (MEM-97) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.