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| Catalog Number | orb1338808 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to Carrier-free (BSA/glycerol-free) mOrange/mOrange2 |
| Target | mOrange |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Immunogen | This antibody is raised against a recombinant protein of mOrange expressed in HEK293 cells. It recognizes both mOrange and mOrange2 proteins. |
| MW | 26.7 kDa |
| Tested applications | WB |
| Dilution range | WB: 1:2000 |
| Application notes | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
| Clone Number | OTI4E10 (formerly 4E10) |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mOrange (Right lane) cDNA for 48 hrs and lysed using Carrier-free (BSA/glycerol-free) antibody
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