MonoMethyl-Histone H3-K79 pAb

• Catalog Number: orb1258181
• Category: Antibodies
• Description: MonoMethyl-Histone H3-K79 pAb
• Target: H3K79me1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Other, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3
• UniProt ID: Q16695
• MW: Observed: 18kDa
• Tested applications: ChIP, IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Research Area: Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K79 antibody (orb1258181). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K79 antibody (orb1258181).

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K79 antibody (orb1258181). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using MonoMethyl-Histone H3-K79 antibody (orb1258181) and rabbit IgG. P1 and P2 were located on GAPDH gene. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.