Monkey IgG (H&L) Antibody Peroxidase

SKU: orb420152

Description

Monkey IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:50,000 - 1:100,000, WB: 1:10,000 - 1:60,000
• Reactivity: Monkey
• Application Notes:
  Anti-Monkey IgG (H&L) HRP conjugated Antibody has been tested in ELISA and western blot and is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Peroxidase, Monkey IgG and Monkey Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit Anti-Monkey IgG peroxidase Conjugated Antibody, rabbit Anti-Monkey IgG Antibody peroxidase Conjugation, rabbit Anti-Monkey IgG HRP Conjugated Antibody

ELISA Results of Rabbit Anti-Monkey IgG Antibody Peroxidase Conjugated tested against purified Monkey IgG HRP. Each well was coated in duplicate with 1.0 µg of Monkey IgG (p/n orb2652733). The working dilution is 1:126000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).