Monkey IgG (H&L) Antibody

SKU: orb420150

Description

Monkey IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:50,000 - 1:100,000, WB: 1:10,000 - 1:60,000
• Reactivity: Monkey
• Application Notes:
  Anti-Monkey IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Monkey IgG and Monkey Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

rabbit anti-Monkey IgG Antibody, Rb-a-Monkey IgG (H&L), Monkey Antibody in rabbit, Monkey IgG Secondary Antibody

Western Blot of Rabbit anti-Monkey IgG antibody. Lane 1: Opal PreStained Molecular Ladder. Lane 2: Monkey IgG (p/n orb2652733). Load: 50 ng per lane. Primary antibody: Monkey IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP (p/n orb347654) secondary antibody at 1:70000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kda for Anti-Monkey IgG.