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Catalog Number | orb420150 |
---|---|
Category | Antibodies |
Description | Monkey IgG (H&L) antibody |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Monkey |
Form/Appearance | Liquid (sterile filtered) |
Concentration | 1.0 mg/ml |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Monkey IgG and Monkey Serum. |
Immunogen | Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit. |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:50,000 - 1:100,000, WB: 1:10,000 - 1:60,000 |
Application notes | Anti-Monkey IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Antibody Type | Secondary Antibody |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Alternative names | rabbit anti-Monkey IgG Antibody, Rb-a-Monkey IgG ( Read more... |
Note | For research use only |
Western Blot of Rabbit anti-Monkey IgG antibody. Lane 1: Opal PreStained Molecular Ladder. Lane 2: Monkey IgG (p/n orb2652733). Load: 50 ng per lane. Primary antibody: Monkey IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP (p/n orb347654) secondary antibody at 1:70000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kda for Anti-Monkey IgG.
FC, FLISA, IF | |
Monkey | |
Goat | |
Polyclonal | |
TRITC |
FC, FLISA, IF, WB | |
Monkey | |
Goat | |
Polyclonal | |
Texas Red |