Monkey IgG (H&L) Antibody Biotin

• Catalog Number: orb420154
• Category: Antibodies
• Description: Monkey IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Monkey
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/ml
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-biotin, Monkey IgG and Monkey Serum.
• Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:50,000 - 1:100,000, WB: 1:5,000 - 1:10,000
• Application notes: Anti-Monkey IgG (H&L) Biotin conjugated Antibody has been tested by ELISA and is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-Monkey IgG biotin conjugated antibody,
• Note: For research use only

ELISA Results of Rabbit Anti-Monkey IgG Antibody Biotin Conjugate tested against purified Monkey IgG Biotin. Each well was coated in duplicate with 1.0 µg of Monkey IgG (p/n orb2652733). The working dilution is 1:31000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).