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Monkey IgG (H&L) Antibody Alkaline Phosphatase
Catalog Number: orb420153
| Catalog Number | orb420153 |
|---|---|
| Category | Antibodies |
| Description | Monkey IgG (H&L) antibody (AP) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | AP |
| Reactivity | Monkey |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-alkaline phosphatase, Monkey IgG and Monkey Serum. |
| Immunogen | Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit. |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000 |
| Application notes | Anti-Monkey IgG (H&L) ALP conjugated Antibody has been tested by ELISA and is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user. |
| Antibody Type | Secondary Antibody |
| Storage | Store secondary antibody at 4° C before opening. DO NOT FREEZE. Alkaline Phosphatase conjugated antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Alternative names | rabbit anti-Monkey IgG alkaline phosphatase conjug Read more... |
| Note | For research use only |
ELISA Results of Rabbit Anti-Monkey IgG Antibody Alkaline Phosphatase Conjugate tested against purified Monkey IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Monkey IgG (p/n orb2652733). The working dilution is 1:4300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Substrate, ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.
