Monkey IgG (H&L) Antibody Alkaline Phosphatase

SKU: orb420153

Description

Monkey IgG (H&L) antibody (AP)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
• Reactivity: Monkey
• Application Notes:
  Anti-Monkey IgG (H&L) ALP conjugated Antibody has been tested by ELISA and is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-alkaline phosphatase, Monkey IgG and Monkey Serum.
• Conjugation: AP

Storage & Handling

• Storage: Store secondary antibody at 4° C before opening. DO NOT FREEZE. Alkaline Phosphatase conjugated antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Monkey IgG alkaline phosphatase conjugated antibody, rabbit anti-Monkey IgG antibody alk phos conjugation

ELISA Results of Rabbit Anti-Monkey IgG Antibody Alkaline Phosphatase Conjugate tested against purified Monkey IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Monkey IgG (p/n orb2652733). The working dilution is 1:4300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Substrate, ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.