MOBKL3 Antibody

• Catalog Number: orb1249216
• Category: Antibodies
• Description: MOBKL3 Antibody
• Target: MOBKL3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-NSNKYFPSRVSIKES
• UniProt ID: Q9Y3A3
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Brain (Frontal Cortex) and in Mouse and Rat Brain lysates (calculated MW of 26.0kDa according to NP_056202.2). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MOBKL3, MOB1, Mps One Binder kinase activator-like
• Note: For research use only
• NCBI: NP_056202.2, NP_955776.1, NP_001094289.1

orb1249216 (0.3 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249216 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249216 (0.3 ug/ml) staining of Mouse Brain lysate (A) and (1 ug/ml) staining of Rat Brain lysate (B) (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.