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Item 1 of 4
Item 1 of 4
MOB4A Antibody (C-term)
Catalog Number: orb1929625
| Catalog Number | orb1929625 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB12538 |
| Tested applications | IF, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 25091 Da |
| Target | This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A. |
| UniProt ID | Q7L9L4 |
| NCBI | NP_001231695.1, NP_775739.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, M Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).MOB4A immunoreactivity is localized to Cytoplasm significantly.
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MOB4A Antibody (C-term) western blot analysis in Daudi, K562 cell line lysates (35 ug/lane). This demonstrates the MOB4A antibody detected the MOB4A protein (arrow).
Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
