MOB2 Antibody

SKU: orb1248872

Description

MOB2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2kDa according to NP_001165694.1). Recommended concentration: 0.02-0.06µg/mL. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: DERGKKVKCTAPQ
• Target: MOB2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MOB2, HCCA2, HCCA2 protein, mob2 homolog, mps one binder kinase activator-like 2

orb1248872 (0.02 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248872 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MOB2

UniProt

Q70IA6

RefSeq (Protein):NP_443731.2, NP_001165694.1