MMP3 Antibody (N-term)

• Catalog Number: orb1788400
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human MMP3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: 30-59 aa
• UniProt ID: P08254
• MW: 53977 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: MMP3; STMY1; Stromelysin-1; Matrix metalloproteina
• Note: For research use only
• NCBI: NP_002413.1

Confocal immunofluorescent analysis of MMP3 Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysates from Raji, HepG2, NCI-H460, Ramos cell line (from left to right), using MMP3 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

MMP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MMP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MMP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MMP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.