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MMP7 Antibody

SKU: orb1247942

Description

MMP7 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in Human Breast, Ovary and Placenta lysates (calculated MW of 29.7kDa according to NP_002414.1). Recommended concentration: 0.03-0.1µg/mL.Immunohistochemistry:In paraffin embedded Human Uterus shows basal staining of an endometrial gland. Recommended concentration, 3-6µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-QKLYGKRSNSRKK
TargetMMP7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

MMP7, matrix metalloproteinase 7 (matrilysin, uterine), HGNC:7174, MMP-7, MPSL1, PUMP-1, matrin, matrix metalloproteinase 7, uterine matrilysin, matrix metallopeptidase 7

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MMP7 Antibody

orb1247942 (0.03 ug/ml) staining of Human Breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MMP7 Antibody

orb1247942 (3.8 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

MMP7 Antibody (orb1247942)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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