MMP-1 rabbit pAb Antibody

SKU: orb765677

Description

Rabbit polyclonal antibody to MMP-1

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MMP-1. AA range:411-460
• Molecular Weight: 54
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-MMP1 antibody, anti-CLG antibody, anti-Interstitial collagenase antibody, anti-Fibroblast collagenase antibody, anti-Matrix metalloproteinase-1 antibody, anti-MMP-1 antibody

Western blot analysis of HELA 293T 3T3 lysis using MMP-1 antibody. Antibody was diluted at 1:1000.

Western Blot analysis of HepG2 cells using MMP-1 Polyclonal Antibody diluted at 1:1000.

Western blot analysis of lysates from 1) K562, 2) Jurkat, 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Monoclonal Antibody (2B8) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using MMP-1 Polyclonal Antibody diluted at 1:1000.