MLL2 Antibody (C-term)

SKU: orb1930201

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:27000, IHC-P-Leica - 1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This MLL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4980-5009 amino acids from the C-terminal region of human MLL2. Antigen Region: 4980-5009 aa.
• Target: KMT2D
• Molecular Weight: 593389 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2, KMT2D, ALR, MLL2, MLL4

Immunohistochemical analysis of paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Anti-MLL2 Antibody (T4720) at 1: 27000 dilution + HMML2 recombinant protein. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-MLL2 Antibody (T4720) at 1: 80000 dilution + recombinant protein whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 593 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

KMT2D

UniProt

O14686

RefSeq (Protein):NP_003473.3