MLCK Antibody (N-term)

SKU: orb1928724

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This MLCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-953 amino acids from the N-terminal region of human MLCK. Antigen Region: 923-953 aa.
• Target: MYLK (HGNC:7590)
• Molecular Weight: 210715 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myosin light chain kinase, smooth muscle, MLCK, smMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1

MLCK Antibody (M1) western blot analysis in SK-BR-3 cell line lysates (35 ug/lane).This demonstrates the MLCK antibody detected the MLCK protein (arrow).

Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.

Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.

Quick Database Links

Gene Symbol

MYLK (HGNC:7590)

UniProt

Q15746

RefSeq (Protein):NP_444260.1, NP_444259.1, NP_444254.3, NP_444256.3, NP_444253.3, NP_444255.3