ML335

SKU: orb1303045

Description

ML335

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 825658-06-8
• MW: 373.25
• Purity: 99.73%
• Formula: C₁₅H₁₄Cl₂N₂O₃S
• SMILES: CS(=O)(=O)Nc1ccc(cc1)C(=O)NCc1ccc(Cl)cc1Cl
• Target: Potassium Channel
• Solubility: 10% DMSO+90% Corn Oil:2 mg/mL (5.36 mM);DMSO:45 mg/mL (120.56 mM)

Bioactivity

• In Vitro:
  Xenopus oocyte two-electrode voltage-clamp measurements indicate that ML335 and ML402 activate K2P2.1 and K2P10.1, but not K2P4.1 (14.3±2.7 μM for K2P2.1-ML335; 13.7±7.0 μM for K2P2.1-ML402; 5.2±0.5 μM for K2P10.1-ML335; and 5.9±1.6 μM for K2P10.1-ML402). Swapping the Lys271 equivalent between K2P2.1 and K2P4.1 results in a reversed phenotype for ML335 and ML402 activation. ML335 and ML402 also activate K2P2.1 in HEK293 cells in a manner similar to their effects in Xenopus oocytes (5.2±0.8 μM and 5.9±1.6 μM for ML335 and ML402, respectively [n≥3]).
• Cell Research:
  Mouse K2P2.1, human K2P4.1, and mutants are expressed from a previously described pIRES2-EGFP vector in HEK293T cells (ATTC). 70% confluent cells are transfected (in 35-mm diameter wells) with LipofectAMINE 2000 for 6 h, and plated onto coverslips coated with Matrigel. Effects of ML335, ML402 and arachidonic acid on K2P2.1 current at 0 mV are measured by whole-cell patch-clamp experiments 24 h after transfection. Acquisition and analysis are performed using pCLAMP9 and an Axopatch 200B amplifier. Pipette resistance ranges from 1 to 1.5 MΩ. Pipette solution contains the following: 145 mM KCl, 3 mM MgCl2, 5 mM EGTA and 20 mM HEPES (pH 7.2 with KOH). Bath solution contains the following: 145 mM NaCl, 5 mM KCl, 1 mM CaCl2, 3 mM MgCl2 and 20 mM HEPES (pH 7.4 with NaOH). K2P2.1 currents are elicited by a 1 s ramp from -100 to +50 mV from a -80 mV holding potential. After stabilization of the basal current, ML335 and ML402 are perfused at 200 mL per hour until potentiation is stably reached.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, ML335, ML-335, ML 335, inhibit, KcsA, Potassium Channel, PotassiumChannel, TREK-1, TREK-2

Compound Identifiers

PubChem CID

1243054