ML264

SKU: orb1300369

Description

ML264 (CID-51003603) is a potent and selective small molecule inhibitor of Kruppel-like factor 5 (KLF5). It effectively suppresses colorectal cancer cell proliferation in vitro and has demonstrated antitumor efficacy in relevant xenograft models, making it a valuable chemical probe for oncology research.

Research Area

Cell Biology, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 1550008-55-3
• MW: 384.88
• Purity: 99.33%
• Formula: C₁₇H₂₁ClN₂O₄S
• SMILES: CN(C1CCS(=O)(=O)CC1)C(=O)CNC(=O)\C=C\c1cccc(Cl)c1
• Target: KLF,DNA/RNA Synthesis
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:71 mg/mL (184.47 mM);10% DMSO+90% Corn Oil:3.3 mg/mL (8.57 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  KLF5:29 nM
• In Vivo:
  In an established xenograft mouse model of colon cancer, ML264 efficiently inhibits growth of the tumor within five days of treatment. this effect is caused by a significant reduction in proliferation and that ML264 potently inhibits the expression of KLF5 and EGR1, a transcriptional activator of KLF5.
• In Vitro:
  ML264 potently halts DLD-1 viability (IC50 = 29 nM) with high maximal effect (>90%). DLD-1 cells are human colorectal adenocarcinoma cells. ML264 has significant effects at submicromolar doses on other cell types as well, including HCT116 (human colorectal carcinoma), HT29 (human colorectal adenocarcinoma), and SW620 (human colorectal adenocarcinoma). The IEC-6 anti-target (a nontransformed rat intestinal epithelial cell line) is largely unaffected, with inhibition below 50% at the highest dose.This compound potently inhibits proliferation of CRC cells in vitro through modifications of the cell cycle profile.
• Cell Research:
  For cell proliferation experiments, DLD-1 and HCT116 cells are treated with 10μM ML264 or with vehicle (DMSO). Live cells are collected at 24, 48 and 72 hours post treatment and their numbers are determined by counting using a Coulter counter; In MTS assay, DLD-1 and HCT116 cells are treated with 10μM ML264 or with vehicle (DMSO). After 24, 48, and 72 hours of incubations, 20 μL of MTS solution is added to each well and an analysis is performed according to the manufacturer's protocol. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNASynthesis, DNA Synthesis, CID51003603, CID-51003603, CID 51003603, Inhibitor, KLF, KLF5, Krüppel-like factor, inhibit, ML 264, ML264, ML-264, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

51003603