MKL1 Antibody

SKU: orb1260831

Description

MKL1 Antibody

Research Area

Cell Biology, Epigenetics & Chromatin, Signal Transduction

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
• Target: MKL1
• Molecular Weight: Observed: 145kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein, Myocardin-related transcription factor A, MRTF-A, MKL1, KIAA1438, MAL

Western blot analysis of extracts of various cell lines, using MKL1 antibody (orb1260831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat brain using MKL1 antibody (orb1260831) (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using MKL1 antibody (orb1260831) (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using MKL1 antibody (orb1260831) (40x lens).

Quick Database Links

Gene Symbol

MKL1

UniProt

Q969V6