MKL1 Antibody

• Catalog Number: orb1260831
• Category: Antibodies
• Description: MKL1 Antibody
• Target: MKL1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
• UniProt ID: Q969V6
• MW: Observed: 145kDa
• Tested applications: IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MKL/myocardin-like protein 1, Megakaryoblastic leu
• Research Area: Cell Biology, Epigenetics & Chromatin, Signal Tran
• Note: For research use only

Western blot analysis of extracts of various cell lines, using MKL1 antibody (orb1260831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat brain using MKL1 antibody (orb1260831) (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using MKL1 antibody (orb1260831) (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using MKL1 antibody (orb1260831) (40x lens).