Mitofusin 2 Rabbit Polyclonal Antibody

• Catalog Number: orb101666
• Category: Antibodies
• Description: Mitofusin 2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Mitofusin 2 (1-120/757aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 83 kDa
• Target: MFN2
• UniProt ID: O95140
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CMT2A; CMT2A2; MARF; CPRP 1; CPRP1; Fzo; HSG; hype
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mitofusin 2) Polyclonal Antibody, Unconjugated (orb101666) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Mitofusin 2 (orb101666) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 83 kD, Observed band size: 68 kD.

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Muscle tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-Mitofusin 2 (orb101666) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 83 kD, Observed band size: 77 kD.

Tissue/cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Mitofusin 2 Polyclonal Antibody, Unconjugated (orb101666) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Mitofusin-2 Polyclonal Antibody, Unconjugated (orb101666) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (orb868805) used at 1:200 dilution for 40 minutes at 37°C.