Mitochondrial Antibody

SKU: orb749993

Description

This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: ELISA (order BSA/sodium azide-free format for coating),Immunofluorescence: 0.5-1ug/ml,Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: AE-1
• Immunogen: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with anti-Mitochondrial antibody (AE-1).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with anti-Mitochondrial antibody (AE-1).

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