MIP-5 (CCL15) Antibody (biotin)

• Catalog Number: orb1671449
• Category: Antibodies
• Description: MIP-5 (CCL15) Antibody (biotin)
• Target: CCL15
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: MIP-5 specific antibody was purified by affinity chromatography and then biotinylated
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-5. Anti-Human MIP-5 specific antibody was purified by affinity chromatography and then biotinylated.
• UniProt ID: Q16663
• Tested applications: ELISA, WB
• Application notes: Sandwich ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5. Western Blot To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Macrophage Inflammatory Protein-5, CCL15, HCC-2, L
• Note: For research use only
• NCBI: Q16663

To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5.

To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.