Mimosine

SKU: orb1300650

Description

L-Mimosine (Leucenol) is a naturally occurring iron-chelating agent and pyridine derivative with antineoplastic properties, originally derived from Leucaena leucocephala. It is widely used in research to study cell cycle arrest, iron metabolism, and has been applied in both in vitro and in vivo models of cancer and dermatology.

Research Area

Cell Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 500-44-7
• MW: 198.18
• Purity: 99.98% (May vary between batches)
• Formula: C₈H₁₀N₂O₄
• SMILES: N[C@@H](Cn1ccc(=O)c(O)c1)C(O)=O
• Target: Others,Apoptosis
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:0.01 mg/mL (0.05 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• In Vivo:
  L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats.
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• In Vitro:
  L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells.
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• Cell Research:
  Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L-Mimosine, iron chelator, iron-binding, NSC 69188, Mimosine, NSC69188, NSC-69188, Leucenine, Leucenol, herbicida, insecticidal, Fe(III), chelator, anti-virus, antiinflammation, anti-influenza, anti-fibrosis

Compound Identifiers

PubChem CID

440473