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Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB25630 |
| Immunogen | This MILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 841-870 amino acids from the C-terminal region of human MILK2. Antigen Region: 841-870 aa. |
| Target | MICALL2 (HGNC:29672) |
| Molecular Weight | 97502 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MICAL-like protein 2, Junctional Rab13-binding protein, Molecule interacting with CasL-like 2, MICAL-L2, MICALL2, JRAB
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MILK2 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MILK2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MILK2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
MILK2 Antibody (C-term) western blot analysis in HL-60, CEM cell line lysates (35 ug/lane). This demonstrates the MILK2 antibody detected the MILK2 protein (arrow).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_891554.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
MILK2 Antibody (C-term) (orb1930772)
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