MIF Antibody

SKU: orb1247265

Description

MIF Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: EIA, ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 5µg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3µg/mL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NAANVGWNNSTFA
• Target: MIF
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor) , HGNC:7097, GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase, macrophage migration inhibitory factor

Quick Database Links

Gene Symbol

MIF

UniProt

P14174

RefSeq (Protein):NP_002406.1