MHC Class II Rabbit Polyclonal Antibody Discontinued

SKU: orb101661

Description

MHC Class II Rabbit Polyclonal Antibody

Research Area

Adaptive Immune System, MHC Class II

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MHC Class II (201-296/296aa)
• Target: HLA-DMB
• Molecular Weight: 29 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HLA-DMB; HLA-DMβ; HLA DMβ; DMA; DMB; DP beta 1; DPB 1; DPB1; DRB; HLA class II histocompatibility antigen DM beta chain; HLA DMB; HLA DPB1; HLADM; HLADP1B; Major histocompatibility complex class II; Major histocompatibility complex class II DP beta 1; MHC class II antigen DMB; MHC DPB1; RING6; RING7; MHC2; HLA class II histocompatibility antigen, DM beta chain.

Tissue/cell: Human pancreatic cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer ( normal goat serum) at 37℃ for 20 min; Incubation: Anti-HLA DMβ Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P of human pancreatic cancer tissue (MHC Class II antibody at 1:200)

Quick Database Links

Gene Symbol

HLA-DMB

UniProt

P28068

Xiaoxuan Lei a 1 , Yu Yang b c 1 , Judun Zheng a , Liwen Liang a , Liuhanghang Cheng b , Yunqing Dong b , Biying Qiu a , Floris J. Bikker e , Tymour Forouzanfar d , Biao Cheng a , Gang Wu f , Bin Yang The cyclization of human salivary Histatin 1 via click chemistry for skin wound healing European Journal of Pharmaceutical Sciences, (2024)

PubMed:

Applications

IF

Reactivity

Mouse

Tavares Pereira, Miguel et al. Selected Uterine Immune Events Associated With the Establishment of Pregnancy in the Dog Front Vet Sci, 7, 625921 (2020)

PubMed: 33634180

Nowak, Marta et al. Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function PLoS One, 13, e0191374 (2018)

PubMed: 29364921

Nowaczyk, Renata M. et al. Cells expressing CD4, CD8, MHCII and endoglin in the canine corpus luteum of pregnancy, and prepartum activation of the luteal TNFα system Theriogenology, 98, 123-132 (2017)

PubMed: 28601149