MHC Class II Rabbit Polyclonal Antibody

• Catalog Number: orb101661
• Category: Antibodies
• Description: MHC Class II Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MHC Class II (201-296/296aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: HLA-DMB
• UniProt ID: P28068
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HLA-DMB; HLA-DMβ; HLA DMβ; DMA; DMB; DP beta 1; DP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xiaoxuan Lei a 1 , Yu Yang b c 1 , Judun Zheng a , Liwen Liang a , Liuhanghang Cheng b , Yunqing Dong b , Biying Qiu a , Floris J. Bikker e , Tymour Forouzanfar d , Biao Cheng a , Gang Wu f , Bin Yang The cyclization of human salivary Histatin 1 via click chemistry for skin wound healing European Journal of Pharmaceutical Sciences, (2024)

PubMed:

Applications

IF

Reactivity

Mouse

Tavares Pereira, Miguel et al. Selected Uterine Immune Events Associated With the Establishment of Pregnancy in the Dog Front Vet Sci, 7, 625921 (2020)

PubMed: 33634180

Nowak, Marta et al. Luteal and hypophyseal expression of the canine relaxin (RLN) system during pregnancy: Implications for luteotropic function PLoS One, 13, e0191374 (2018)

PubMed: 29364921

Nowaczyk, Renata M. et al. Cells expressing CD4, CD8, MHCII and endoglin in the canine corpus luteum of pregnancy, and prepartum activation of the luteal TNFα system Theriogenology, 98, 123-132 (2017)

PubMed: 28601149

Tissue/cell: Human pancreatic cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer ( normal goat serum) at 37℃ for 20 min; Incubation: Anti-HLA DMβ Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P of human pancreatic cancer tissue (MHC Class II antibody at 1:200)