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MGP polyclonal antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB 1:500 - 1:2000 IHC1:100 - 1:200 IF/ICC 1:50 - 1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human MGP(NP_000891.2). |
| Molecular Weight | 12KDa |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of extracts of SH-SY5Y cells, using MGP polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 180s.

IHC of paraffin-embedded human breast cancer using MGP polyclonal antibody at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC of paraffin-embedded human breast cancer using MGP polyclonal antibody at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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UniProt Details
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Protocol Information
MGP polyclonal antibody (orb642281)
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