MFAP4 Antibody (C-term)

• Catalog Number: orb1930751
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This MFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the C-terminal region of human MFAP4.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P55083
• MW: 28648 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50, WB: 1:1000
• Antibody Type: Primary Antibody
• Clone Number: RB25626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Microfibril-associated glycoprotein 4, MFAP4
• Note: For research use only
• NCBI: NP_001185624.1, NP_002395.1

Western blot analysis of MFAP4 Antibody (C-term) in mouse lung tissue lysates (35 ug/lane). MFAP4 (arrow) was detected using the purified Pab.

Western blot analysis of lysate from human lung tissue lysate, using MFAP4 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.

MFAP4 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MFAP4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFAP4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.