METTL3 Rabbit pAb Discontinued

SKU: orb374082

Description

Rabbit polyclonal antibody to METTL3

Research Area

Cancer, DNA Damage, Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:100-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2).
• Molecular Weight: 70kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IME4, M6A, MT-A70, Spo8, hMETTL3, METTL3

Confocal immunofluorescence analysis of Hela cells using METTL3 Rabbit pAb (orb374082) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using METTL3 Rabbit pAb (orb374082) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-3 cells using METTL3 Rabbit pAb (orb374082) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using METTL3 Rabbit pAb (orb374082) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using METTL3 Rabbit pAb (orb374082) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western blot analysis of lysates from wild type (WT) and METTL3 knockdown (KD) 293T cells using METTL3 Rabbit pAb (orb374082) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of various lysates using METTL3 Rabbit pAb (orb374082) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Quick Database Links

UniProt

Q86U44

Xiaodong Yu, Yaxun Li, Fanshuang Kong, Qun Xu METTL3 regulates FAM83D m6A modification to accelerate tumorigenesis of triple-negative breast cancer via the Wnt/β-catenin pathway Toxicol In Vitro, 105746 (2023)

PubMed: 38043628

Applications

WB

Reactivity

Human

Condic, Mateja et al. N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma BMC Cancer, 22, 943 (2022)

PubMed: 36050747

Applications

IHC

Reactivity

Human