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Catalog Number | orb1260839 |
---|---|
Category | Antibodies |
Description | METTL13 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human METTL13 (NP_057019.3). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 85kDa |
Target | METTL13 |
UniProt ID | Q8N6R0 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | KIAA0859, 5630401D24Rik, CGI-01, FLJ10310, feat, K Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of MCF7 cells, using METTL13 antibody (orb1260839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using METTL13 antibody (orb1260839) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using METTL13 antibody (orb1260839) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using METTL13 antibody (orb1260839). Blue: DAPI for nuclear staining.
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