Methylated Lysine Antibody (Biotin)

SKU: orb67535

Description

Rabbit polyclonal to Methylated Lysine(Biotin). Detects proteins containing methylated lysine residues in SDS PAGE immunoblots.

Research Area

Epigenetics & Chromatin, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	ELISA, ICC, IF, IP, WB
Dilution range	WB (1:5000), ICC/IF (1:50)
Reactivity	All
Application Notes	0.2-0.5 µg/ml of SPC-159 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Methylated KLH Conjugated
Target	Methylated Lysine
Purification	Protein A Purified
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Concentration	0.25 mg/ml
Disclaimer	For research use only

Alternative Names

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Methylated Lysine, Methyl lysine, Dimethylysine, Trimethyl lysine, N epsilon dimethyl lysine

Quality Guarantee

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Methylated Lysine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite.

Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right).

Quick Database Links

Gene Symbol

Methylated Lysine

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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