Methotrexate metabolite

SKU: orb1308047

Description

DAMPA (7-hydroxymethotrexate) is the primary active metabolite of the antifolate chemotherapeutic and immunosuppressant Methotrexate. It is widely used in pharmacological research to study drug metabolism, mechanisms of action, and resistance in both in vitro and in vivo cancer and autoimmune disease models.

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 19741-14-1
• MW: 325.33
• Purity: 95.28% (May vary between batches)
• Formula: C₁₅H₁₅N₇O₂
• SMILES: CN(Cc1cnc2nc(N)nc(N)c2n1)c1ccc(cc1)C(O)=O
• Target: Antifolate|||Drug Metabolite
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (3.07 mM);DMSO:4 mg/mL (12.3 mM)

Bioactivity

• In Vitro:
  Methotrexate metabolite was the most active, with 50% inhibitory concentrations in vitro of 446 ng/ml against the antifolate-sensitive strain and 812 ng/ml against the highly resistant strain under physiological folate conditions. Methotrexate metabolite potentiates the activity of the sulfone dapsone, an inhibitor of dihydropteroate synthase, but not that of chlorcycloguanil, a known inhibitor of dihydrofolate reductase (DHFR).
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Drug Metabolite, DrugMetabolite, DAMPA, Antifolate, Methotrexate metabolite, Inhibitor, inhibit

Compound Identifiers

PubChem CID

72441