Methacycline hydrochloride

SKU: orb1310534

Description

Methacycline hydrochloride is a semisynthetic tetracycline antibiotic with a broad spectrum of activity and an extended half-life, leading to prolonged effective blood concentrations. It is utilized in research for studying bacterial protein synthesis inhibition in both in vitro assays and in vivo infection models.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 3963-95-9
• MW: 478.88
• Purity: 99.71% (May vary between batches)
• Formula: C₂₂H₂₃ClN₂O₈
• SMILES: Cl.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@@H]3C(=C)c4cccc(O)c4C(=O)C3=C(O)[C@]2(O)C(=O)C(C(N)=O)=C1O
• Target: Antibiotic,ribosome,Antibacterial
• Solubility: 10% DMSO+90% Saline:2 mg/mL (4.18 mM);DMSO:27.5 mg/mL (57.43 mM)

Bioactivity

• In Vivo:
  Methacycline inhibits the TGF-β1-induced non-Smad signaling pathways, including the activation of c-Jun N-terminal kinase (JNK), p38, and Akt, without suppressing Smad or β-catenin transcriptional activities. It does not affect the baseline activities of JNK, p38, or Akt, nor the TGF-β1 response of lung fibroblasts. Additionally, methacycline inhibits the TGF-β1-induced expression of α-smooth muscle actin (α-SMA), SNAIL1, and type I collagen in primary alveolar epithelial cells.
• In Vitro:
  Methacycline, within pulmonary alveolar macrophages, does not alter the response gene of TGF-β1 nor attenuates the aggregation of inflammatory cells. Following tracheal aspiration of bleomycin, intraperitoneal injection of Methacycline at 100 mg/kg, starting on day 10, enhances survival rates by day 17. Methacycline mitigates bleomycin-induced classical EMT (Epithelial-Mesenchymal Transition) markers, including SNAIL1, TWIST1, type I collagen, fibronectin, and their mRNA expressions.

Storage & Handling

• Storage: keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p38, Rondomycin, pulmonary, Smad, TGF-β1, transition, c-Jun, Bacterial, Antibiotic, 16S ribosome, 30S ribosome, Akt, fibrogenesis, epithelial-mesenchymal, EMT, fibrosis, Inhibitor, N-terminal, Methacycline, Methacycline HCl, Methacycline hydrochloride, Methacycline Hydrochloride, kinase, inhibit

Compound Identifiers

PubChem CID

54685047