Methacycline hydrochloride

SKU: orb1225116

Description

Methacycline hydrochloride is a tetracycline antibiotic, and also an inhibitor of epithelial–mesenchymal transition (EMT) with IC50 of roughly 5 μM, used to treat various infections.(In Vitro):Methacycline hydrochloride is an inhibitor of A549 EMT with an IC50 of roughly 5 μM.In vitro, Methacycline hydrochloride (10, 20 μM; for 48 hours) inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline hydrochloride inhibits TGF-β1–induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1.\n(In Vivo):In vivo, Methacycline hydrochloride (100 mg/kg/day; ip; beginning 10 days after intratracheal Bleomycin) improves survival at Day 17. Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA, ARE attenuated by Methacycline hydrochloride (Day 17).

Key Properties

• CAS Number: 3963-95-9
• MW: 478.88
• Purity: >98% (HPLC)
• Formula: C₂₂H₂₃ClN₂O₈
• SMILES: O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])C(C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)=C)N.[H]Cl
• Target: ribosome
• Solubility: Water: 5 mg/mL (10.44 mM); DMSO: 3 mg/mL (6.26 mM)

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

6-methylene Oxytetracycline | Rondomycin

Quick Database Links

Gene Symbol

ribosome