MSLN Antibody (Biotin)

SKU: orb751142

Description

Mouse monoclonal antibody against Mesothelin conjugated to Biotin

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC
• Dilution range: Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Positive Control: CAPAN-2 or HeLa cells. Lung Mesothelioma

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: MSLN/2131
• Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)
• Target: MSLN
• Source/Expression System: Bioreactor
• Molecular Weight: 40kDa (Mature form); 69kDa (Precursor)
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Form/Appearance: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. Contact us if you require this Ab in a different format.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-CAK1 antibody, anti-Megakaryocyte potentiating factor antibody, anti-Mesothelin antibody, anti-MSLN antibody, anti-SMR antibody, anti-SMRP antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using Mesothelin Mouse Monoclonal Antibody (MSLN/2131) (unconjugated). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Biotin-conjugated Mesothelin Mouse Monoclonal Antibody (MSLN/2131). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lung stained with Biotin-conjugated Mesothelin Mouse Monoclonal Antibody (MSLN/2131). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified Mesothelin Mouse Monoclonal Antibody (MSLN/2131) (unconjugated). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

MSLN

UniProt

Q13421

NCBI Gene ID

10232