Merlin Antibody (N-term)

• Catalog Number: orb1929811
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Merlin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the N-terminal region of human Merlin.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P35240
• MW: 69690 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB19187
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Merlin, Moesin-ezrin-radixin-like protein, Neurofi
• Note: For research use only
• NCBI: NP_861971.1, NP_000259.1, NP_861970.1, NP_057502.2, NP_861546.1, NP_861968.1, NP_861969.1, NP_861967.1, NP_861966.1

Confocal immunofluorescent analysis of Merlin Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Merlin Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Merlin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of Merlin Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). NF2 (arrow) was detected using the purified Pab.