Melanoma Marker (MART-1 + Tyrosinase + gp100) antibody

• Catalog Number: orb607989
• Category: Antibodies
• Description: Mouse monoclonal antibody to Melanoma Marker (MART-1 + Tyrosinase + gp100)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DT101+ BC199 + T311 + HMB45
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse / IgG2b, kappa + Mouse / IgG1, kappa + Mouse / IgG2a, kappa
• Immunogen: Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45); Recombinant tyrosinase protein (T311)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Conjugation: Unconjugated
• MW: 20-22kDa (doublet) (MART-1): 90-100kDa (gp100); 70-80kDa (Tyrosinase)
• Entrez: 2315, 6490
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Melanoma antigen recognized by T-cells 1 (MART-1),
• Note: For research use only
• Expiration Date: 12 months from date of receipt.