Melanoma Antibody [PNL2]

SKU: orb1252406

Description

Melanoma Antibody [PNL2]

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Reactivity: Canine, Human, Mouse
• Application Notes:
  Immunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: PNL2
• Immunogen: Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma antibody.

SDS-PAGE Analysis of Purified, BSA-Free Melanoma Antibody (clone PNL2). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

NotKnown