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Item 1 of 3
MEK3 (MAP2K3) Antibody (N-term)
Catalog Number: orb1928768
| Catalog Number | orb1928768 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This MEK3 (MAP2K3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human MEK3 (MAP2K3). |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P46734 |
| MW | 39318 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100 |
| Antibody Type | Primary Antibody |
| Clone Number | RB0928 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Dual specificity mitogen-activated protein kinase Read more... |
| Note | For research use only |
| NCBI | NP_002747.2, NP_659731.1 |
Western blot analysis of anti-MKK3 Pab in Jurkat cell lysate. MKK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IHC of matched glioma core (top row) and rim (middle row) samples as well as normal brain control (bottom row) from a glioma invasion specific tissue microarray. Insets, individual tumor cells (black squares) are presented at higher magnification to facilitate interpretation of the staining pattern. Staining for MKK3 and pMKK3 exhibits strong signal in majority of invasive cells (B) compared with stationary cells from tumor core (A). Normal brain control shows only weak staining in reactive astrocytes (C).
